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fred gage  (Addgene inc)


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    Addgene inc fred gage
    Fred Gage, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fred+gage/pLVX-EF1alpha-2xGFP%3ANES-IRES-2xRFP%3ANLS+(Plasmid+%2371396)/pmc12548433-164-8-12
    Average 93 stars, based on 13 article reviews
    fred gage - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Methods for Mitochondrial DNA Damage and Depletion in Immortalized Trabecular Meshwork Cells
    Article Snippet: .. For all plasmids used, plasmid maps are accessible from Addgene using the identifiers provided. pLVX-EF1a-tetOn-IRES-G418 (EtO) was a gift from Fred Gage (Addgene plasmid # 84776), pMA3287 was a gift from Mikhail Alexeyev (Addgene plasmid # 46883), and psPAX2 and pMD2.G were gifts from Didier Trono (Addgene plasmid # 12260 and # 12259). ..

    Article Title: Lamin B1 overexpression alters chromatin organization and gene expression.
    Article Snippet: .. The pLVXTP plasmid backbone used for stable cell-line production was derived from the pLVXUbC-rtTA-Ngn2:2A:EGFP plasmid, a gift from Fred Gage (Addgene plasmid # 127288; http:// n2t.net/addgene:127288; RRID:Addgene_127288) [36]. .. The Ngn2:2A:EGFP insert was removed using Not1 and EcoR1 restriction enzymes, and In-Fusion cloning was used to insert mCherry, or mCherry tagged LmnB1 (NM_005573), LmnB1ΔCaaX (NM_005573 with a four amino acid deletion (Cys, Ala, Ile, and Met) prior to the stop codon), or Lap2β (NM_001032283).

    Article Title: The leader proteins of Theiler’s virus and Boone cardiovirus use a combination of short linear motifs (SLiMs) to target RSK kinases to the nuclear pore complex
    Article Snippet: Expression plasmids were derived from pcDNA3 (Invitrogen). .. The lentiviral construct pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS was a gift from Fred Gage ( ) (Addgene plasmid # 71396). ..

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H

    Stable Transfection:

    Article Title: Lamin B1 overexpression alters chromatin organization and gene expression.
    Article Snippet: .. The pLVXTP plasmid backbone used for stable cell-line production was derived from the pLVXUbC-rtTA-Ngn2:2A:EGFP plasmid, a gift from Fred Gage (Addgene plasmid # 127288; http:// n2t.net/addgene:127288; RRID:Addgene_127288) [36]. .. The Ngn2:2A:EGFP insert was removed using Not1 and EcoR1 restriction enzymes, and In-Fusion cloning was used to insert mCherry, or mCherry tagged LmnB1 (NM_005573), LmnB1ΔCaaX (NM_005573 with a four amino acid deletion (Cys, Ala, Ile, and Met) prior to the stop codon), or Lap2β (NM_001032283).

    Derivative Assay:

    Article Title: Lamin B1 overexpression alters chromatin organization and gene expression.
    Article Snippet: .. The pLVXTP plasmid backbone used for stable cell-line production was derived from the pLVXUbC-rtTA-Ngn2:2A:EGFP plasmid, a gift from Fred Gage (Addgene plasmid # 127288; http:// n2t.net/addgene:127288; RRID:Addgene_127288) [36]. .. The Ngn2:2A:EGFP insert was removed using Not1 and EcoR1 restriction enzymes, and In-Fusion cloning was used to insert mCherry, or mCherry tagged LmnB1 (NM_005573), LmnB1ΔCaaX (NM_005573 with a four amino acid deletion (Cys, Ala, Ile, and Met) prior to the stop codon), or Lap2β (NM_001032283).

    Expressing:

    Article Title: Altered theta and beta oscillatory synchrony in a genetic mouse model of pathological anxiety
    Article Snippet: .. To locally delete Nlgn2 using local expression of Cre recombinase, AAV5 vectors were generated using the AAV‐GFP and AAV‐GFP/Cre plasmids generously provided by Fred Gage (Addgene plasmids 49055 and 49056, Watertown, MA, USA) in combination with the pDP5rs packaging system (PlasmidFactory, Bielefeld, Germany) as previously described. ..

    Generated:

    Article Title: Altered theta and beta oscillatory synchrony in a genetic mouse model of pathological anxiety
    Article Snippet: .. To locally delete Nlgn2 using local expression of Cre recombinase, AAV5 vectors were generated using the AAV‐GFP and AAV‐GFP/Cre plasmids generously provided by Fred Gage (Addgene plasmids 49055 and 49056, Watertown, MA, USA) in combination with the pDP5rs packaging system (PlasmidFactory, Bielefeld, Germany) as previously described. ..

    Bioprocessing:

    Article Title: Altered theta and beta oscillatory synchrony in a genetic mouse model of pathological anxiety
    Article Snippet: .. To locally delete Nlgn2 using local expression of Cre recombinase, AAV5 vectors were generated using the AAV‐GFP and AAV‐GFP/Cre plasmids generously provided by Fred Gage (Addgene plasmids 49055 and 49056, Watertown, MA, USA) in combination with the pDP5rs packaging system (PlasmidFactory, Bielefeld, Germany) as previously described. ..

    Construct:

    Article Title: The leader proteins of Theiler’s virus and Boone cardiovirus use a combination of short linear motifs (SLiMs) to target RSK kinases to the nuclear pore complex
    Article Snippet: Expression plasmids were derived from pcDNA3 (Invitrogen). .. The lentiviral construct pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS was a gift from Fred Gage ( ) (Addgene plasmid # 71396). ..

    Adhesive:

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H

    Electron Microscopy:

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H

    Hybridization:

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H

    Amplification:

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H

    Recombinant:

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H

    Software:

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H

    Injection:

    Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H



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